Protocols

Histology and Sectioning

Fixation – PDF
Paraffin embedding/sectioning
OCT embedding/sectioning
H&E Staining for OCT sections – PDF
H&E Staining for paraffin sections
Immunohistochemistry – PDF
gDNA Extraction from sections – PDF
Laser Capture Microscopy – PDF

RNA

Homogenizing Tissues and RNA Extraction – PDF
Agilent BioAnalyzer
RNA Amplification

qPCR and Microarray

Agilent Array – PDF
RT cDNA sysnthesis – PDF

DNA

Ethanol precipitation of DNA
Sequencing – PDF
gDNA from sections
gDNA from tissue
PCR
Tail DNA prep

Protein

Tissue homogenization and protein extraction
Western blotting

Cell Culture

Passaging cell lines in mice – PDF

Mouse Protocols

Colony maintenance
Necropsy
Sample necropsy form
Tail DNA Prep
Xenogen imaging
Procedures

Misc

Solution recipes
Product inserts